Coding

Part:BBa_K4139017:Design

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-26)


CAP-FP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 874
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 874
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1056
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 874
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 874
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A bi-specific chimeric protein designed to interact with PbEL04 and PRO1 tagged with GFP as fluorescent signal.

Source

Aequorea victoria

References

Feng, J., Hwang, R. U., Hwang, S. F., Strelkov, S. E., Gossen, B. D., Zhou, Q. X., & Peng, G. (2010). Molecular characterization of a serine protease Pro1 from Plasmodiophora brassicae that stimulates resting spore germination. Molecular Plant Pathology, 11(4), 503–512. https://doi.org/10.1111/j.1364-3703.2010.00623.x

Jiang, X., Su, Y., & Wang, M. (2022). A small cysteine-rich protein identified from the Proteome of clubroot pathogen, Plasmodiophora brassicae, induces cell death in nonhost plants and host plants. https://doi.org/10.21203/rs.3.rs-1961445/v1

Lowe, D. G., Ricketts, M., Levinson, A. D., & Goeddelt, D. V. (1988). Chimeric proteins define variable and essential regions of Ha-ras-encoded protein (guanine nucleotide-binding protein/Ha-ras p21/R-ras p23/mammalian transformation). In Proc. Nail. Acad. Sci. USA (Vol. 85).

Valdivia, R. H., Hromockyj, A. E., Monack, D., Ramakrishnan, L., & Falkow, S. (1996). Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions *. In Gene (Vol. 173).